Quantitative Analysis of Cu(I) Concentration in Click Chemistry — Biotinylation at Side Chain of Propargylglycine Using Click Chemistry under Heating Conditions —

Specific and quantitative labeling of biomolecules using click chemistry

Specific and highly efficient fluorescent labeling techniques for biomolecules, especially for proteins, are required for the quantitative analyses of bio-phenomena and for subsequent systems biology. Although expression of exogenous proteins fused with fluorescent tags, such as green fluorescent protein, is the most widely used method for quantitative bio-analysis, the following problems need ...

Surface Functionalization of Exosomes Using Click Chemistry

A method for conjugation of ligands to the surface of exosomes was developed using click chemistry. Copper-catalyzed azide alkyne cycloaddition (click chemistry) is ideal for biocojugation of small molecules and macromolecules to the surface of exosomes, due to fast reaction times, high specificity, and compatibility in aqueous buffers. Exosomes cross-linked with alkyne groups using carbodiimid...

Functionalization of polymer microspheres using click chemistry.

We describe a new method to covalently link a wide variety of molecules to the surface of colloidal polymer microspheres using the Cu(I)-catalyzed azide-alkyne reaction, most commonly known under the class of reactions identified by the term click chemistry. The method is generic and readily applied to a spectrum of colloidal particle systems allowing surfaces to be tailored with virtually any ...

Click chemistry with DNAw

Click Chemistry background information

Especially biomolecule labeling requires reaction procedures that can be performed under physiological conditions (neutral pH, aqueous solution, ambient temperature) with low reactant concentrations to ensure non-toxic, low background labeling at reasonable time scales while still preserving biological function. Among the plethora of possible reactions only a few generally fit the necessary rea...